Coding
Mnt opt

Part:BBa_K566008:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)

Mnt repressor optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and removed.


Source

Original sequence was taken from BioBrick BBa_C0072, this was optimized with preferential codons for better expression into E. coli.

References

1. Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. J. Mol. Riol. (1987) 195, 311-322