Coding
Mnt opt
Part:BBa_K566008:Design
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-26)
Mnt repressor optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 228
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites was performed, they were found and removed.
Source
Original sequence was taken from BioBrick BBa_C0072, this was optimized with preferential codons for better expression into E. coli.
References
1. Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. J. Mol. Riol. (1987) 195, 311-322